Top : Science : Biology : Biochemistry and Molecular Biology :
Methods and Techniques
Categories
| Arrays Cell Biology Methods @ Immunochemical Methods @ Molecular Modeling @ PCR |
Websites
Collection of molecular biology techniques derived from help files of Dr. Harold Zappe at the Department of Medical Microbiology, University of Cape Town, South Africa.
http://www.uct.ac.za/depts/mmi/bbhelp/intro.html
Description of methods behind gene cloning, genetic engineering, DNA recombination and isolation, including discussion of restriction enzymes and ligase.
site exerpt
Methods in genetic sequencing, DNA analysis and gene cloning All text and information in the format below is copyright under the laws of the United Kingdom and other countries. The whole text or complete sections must not be copied verbatim,...Forum for exchange of views on the single cell gel electrophoresis system, capable of detecting single- and double-strand breaks, oxidative DNA base damage, and DNA, protein and drug crosslinking. Includes related links.
site exerpt
Comet Assay Interest Group Comet assay is a rapid and very sensitive fluorescent microscopic method to examine DNA damage and repair at individual cell level. This assay has critical applications in fields of toxicology ranging from aging and clinical investigations to genetic toxicology and...Directory of molecular biology protocol links.
site exerpt
SciWeb The Life Science Home Page S enter whole or partial words, numbers, or strings in the company or product name, CAS, or NSN (grep Find: It is ideal to enter one word from the company name and one word from the product name, i.e baker...Richard Man-kit Yu's site where you can acquire various molecular biology techniques.
site exerpt
MolBio Spot Protein sequence analyses (i) Most popular servers NCBI National Centre for Biotechnology Information EMBL-EBI European Bioinformatics Institute BCM Search Launcher by Baylor College of Medicine ExPASy Molecular Biology Server Expert Protein Analysis System (ii) Sequence homology analaysis BLAST (NCBI) Align...Different methods of determining protein concentration, as a resource for researchers and students in biochemistry and molecular biology laboratories from University of Nebraska, Lincoln, NE.
http://www-class.unl.edu/biochem/protein_assay/
Handbook of fluorescence applications, bibliography, data tables and calculators for molecular biology, information on courses and workshops from supplier in Eugene, OR and Leiden, Netherlands.
http://www.probes.com/resources/
Methods, information, programs and BioMail for molecular biologists, as joint venture between Germany and Russia.
http://molbiol.ru/eng/
Subscription only source of laboratory techniques in DNA and protein science, to complement the hard copy, giving scientists the benefit of both media from CSHL, NY.
site exerpt
Cold Spring Harbor Laboratory Press A new paradigm in science publishing 149; Searchable Protocols 149; Frequent Updates 38; Additions 149; Easy-Print Protocols 149; The Print/Online Advantage A quick look at our online manual Access to step-by-step instructions for members, abstracts for guests Receive e-mail notification...Resource for molecular biologists on methods, with feedback for user-directed evolution and links to commercial suppliers from Jonathan D Rees, Oxford, UK.
http://www.molbiol.net/
CGH is a molecular cytogenetic method of screening a tumor for genetic changes. CGH protocols, images, publications, events and links maintained by the University Hospital Charité, Humboldt-University of Berlin, Germany.
site exerpt
Comparative Genomic Hybridization (CGH) H is a molecular cytogenetic method of screening a tumor for genetic changes. The alterations are classified as DNA gains and losses and reveal a characteristic pattern that includes mutations at chromosomal and subchromosomal levels. CGH Images CGH Protocols CGH...Collection of laboratory protocols, applications and links to publications in biomedicine and molecular biology from author in Germany.
http://www.biomedpage.de/indexe.html
Online calculations of nucleic acid extinction coefficients, melting temperatures, and links to biophysics databases. Includes thesis, publications and contacts for commercial scientist in Coralville, Iowa.
site exerpt
DNA thermodynamics melting temperature (Tm) and extinction coefficient Richard Owczarzy I investigate biophysics of DNA and RNA using experimental and theoretical approaches. My research is focused on the stability and thermodynamics of various structures of nucleic acids, including duplexes, hairpins, bulges, dumbbells, junctions, and molecular beacons. I develop models that...Calculates molecular weight, composition and concentration of nucleic acids and proteins, based on provided sequence, dilution and absorbance measurement. Also melting temperatures for nucleic acids. Maintained at Schepartz Laboratory of Chemical Biology, Yale, New Haven, CT.
site exerpt
Biopolymer Calculator Click here to go to the frameless calculator....Protocol collection, with interactive forum for over ten topics, covering DNA, PCR, RNA and proteins techniques and links to online journals and bioinformatics resources, from the Northwest Fisheries Science Center Microbial Pathogenesis Lab, Seattle, WA.
http://micro.nwfsc.noaa.gov/protocols/
Internet project initiated in early 1992 as BRNI, bio.com includes PCR, hybridization and microarray protocols.
site exerpt
BioProtocol: Biotechnology Pharmaceutical Laboratory Research Protocols Biotech, Pharma, Biomedical, Life Science Protocols contributed by scientists from over 125 academic laboratories World renowned Editorial Board Wide variety of life science disciplines covered Searchable protocol database Printable versions available for all protocols Store protocols of particular interest to you and send protocols to...The use of DNA techniques in phylogenesis. Explanations, protocols and animations from Andy Vierstraete at the University of Ghent, Belgium.
http://allserv.ugent.be/~avierstr/
Collection of DNA microarray technology protocols, primarily for resident students at Stanford.
site exerpt
Surgery and Surgical Research Unauthorized use of this site is unlawful 1999...Teaching manual to support the applied biotechnology programs at the University of Maryland, Baltimore.
http://www.research.umbc.edu/~jwolf/method1.html
Preparing probe, cloning into plasmids, complementarity and hybridization, gel electrophoresis, transfer to solid support and blotting variants, blocking, target detection PCR problems from chapter in MIT hypertext handbook.
site exerpt
Recombinant DNA Chapter Directory Practice problems hyperbio@mit.edu...Collection of protocols, maps and sequences regarding manipulation of lentiviral vectors, siRNA and transgenic animals. Applications in HIV, stem cell and gene therapy research at University of Geneva.
site exerpt
There are new sections coming soon about lentivector mediated transgenesis and stem cells NEW Due to large number of requests, please allow at least 3 weeks for plasmid shipment. In order to avoid further delays: Please send us both MTA...Multiple alignment and dynamic evolutionary conservation profile of several vertebrate genomes including human, mouse, rat, chicken, frog, and three fishes, developed at LLNL, Livermore, California.
site exerpt
ECR Browser Browser is a dynamic whole-genome navigation tool for visualizing and studying evolutionary relationships between vertebrate and non-vertebrate genomes. The tool is constantly being updated to include the most recently available sequenced genomes (currently: human, dog, mouse, rat, chicken, frog two...Description of an agarose gel set-up. Includes instructions for camera operation with a script, with computer side image analysis from the University of Virginia, Charlottesville.
http://www.virginia.edu/biology/Fac/hirsh_gel/gel/
Microsatellites and others techniques from the Savana River Ecology Laboratory, University of Georgia.
http://www.uga.edu/srel/DNA_Lab/protocols.htm
Overview of genotyping, detection and quantifying single nucleotide polymorphism, including the application of template directed dye terminator incorporation assay. Offers database and links to other resources from PerkinElmer Life Sciences, Boston, MA.
http://las.perkinelmer.com/catal...ry.aspx?CategoryName=SNP+Scoring
Alphabetical list for buffer recipes, including constituents for cultures, from a personal page in Switzerland.
site exerpt
Reto's Buffer Media Book Inhibits cell wall synthesis by inhibiting formation of the peptidoglycan crosslink b-lactamase (286 aa) is secreted into the periplasm and cleaves lactam antibiotic Chloramphenicol 20 Inhibits protein synthesis by interacting with the 50S ribosomal subunit and inhibiting the peptidyltransferase reaction....Resources such as polymerase chain reaction, dedicated to method development. Part of internet directory maintained by Horizon Scientific Press, Wymondham, England.
site exerpt
Molecular Biology Protocols A comprehensive collection of molecular biology protocols, molecular biology methods, molecular biology techniques, molecular biology procedures, molecular biology strategies, general laboratory protocols, laboratory techniques, laboratory methods, and procedures. Sponsored by the following companies Brinkmann Instruments offers many Eppendorf products for...